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A total of 95 BAC clones (14.2%) failed to produce distinct and reproducible FISH signals, even after multiple hybridization attempts.
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While multiple hybridization replicates provided ∼97% accuracy, even a single replicate provided ∼95% accuracy.
Despite repeated hybridization attempts, no data were obtained in one case.
However, the GeneChip platform offers multiple probes per gene, potentially offsetting the need for longer probes through multiple hybridization points.
In addition multiple hybridization probes for one gene were condensed into a single event.
Technical replicates involved multiple hybridizations of single RNA preparations.
These variants were validated across multiple hybridizations and also via PCR.
This biological resource was basically produced by multiple hybridizations of local cultivars and irradiation exposure [ 14].
Sharon's made multiple suicide attempts over the years.
Musharraf survived multiple assassination attempts while he was in office.
Eleven case records indicated the need for multiple intubation attempts.
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