Sentence examples for multiple housekeeping genes from inspiring English sources
Multiple housekeeping genes (usually seven genes) are examined in the analysis as their sequences are constrained and variations are nearly neutral.
mRNA expression was calculated as a copy number (Ct) value and normalized using multiple housekeeping genes (B2M, RPL13A, GAPDH, ΔCt).
This analysis, therefore, confirms the increased utility of multiple housekeeping genes for studies of the geographic population structure of LB group spirochetes and suggests an association between the population structure of the bacteria and that of their vertebrate hosts.
CNRQ values were calculated by classic delta-delta-Ct method and normalized to the multiple housekeeping genes, cadherin-associated protein, beta 1 CTNNB1) and GAPDH using the qBasePlus 1.3 analysis software.
Based on sequence analyses of multiple housekeeping genes (i.e. clpA, clpX, nifS, pepX, pyrG, recG and rplB) of the B. lusitaniae samples analyzed in this study (Table 1), 13 sequence types (STs) were defined by MLST, and no ST was observed in more than two samples (Table 2).
Each sample was assayed in duplicate, and CNRQ (calculated normalized relative quantity) values were calculated by classic delta-delta-Ct method and normalized to the multiple housekeeping genes, large, P0 (RPLPO) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) as a control using the qBasePlus 1.3 analysis software (http://www.biogazelle.com) [44].
By analyzing multiple housekeeping gene loci, strains with related phylogenetic background can be grouped and analyzed together [ 20].
The GEArray expression analysis software was utilized to enable background normalization, correction for different degrees of exposure, and normalization with multiple housekeeping gene controls on each membrane.
The evidence of overexpression of multiple housekeeping RBR genes after geminivirus infection [28], described above, is a first hint of what may be a promising line of research.
The M values for the snoRNAs were higher than expected: for example, in a study of multiple mRNA housekeeping genes, Vandesompele et al (2002) typically found when three housekeeping genes were measured that M values were less than 0.5.
Relative expression was analysed with Applied Biosystems StepOne, using the ΔΔCT method corrected for amplification efficiency [ 50] and following [ 51] for applying multiple reference (housekeeping) genes: EF1d/b (elongation factor-1 B beta) and SUMO3 (small ubiquitin-like modifier).
