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When silenced, the decrease of phosphoethanolamine N-methyltransferase produced not only multiple growth defects but also temperature sensitive male sterility in Arabidopsis[ 49].
Mutations in both proteins cause multiple growth defects when cells were cultured in low-quality amino acid conditions (Neklesa and Davis, 2009).
The most successful trial in terms of deletion size was, however, performed via insertion and excision in an untargeted approach, but resulted in multiple growth defects of the constructed strains [ 15].
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Deletion of efr3 also led to multiple growth sensitivities and defects in cell division.
As an example, in S. cerevisiae, overexpression of SRO7 (an Lgl family protein that interacts with t-SNAREs) can suppress the growth defects of multiple exocyst mutants [ 102].
Fourth, multiple mutations result in combinatorial growth defects with slx5Δ (Pan et al. 2006; Wang et al. 2006), but many of these genes do not encode ubiquitin E3s (AOSMT3SMT3, CCR4, and many others).
Perturbing gpb-1 and lin-5 induced growth defects in multiple strains such that the parental generation of worms failed to develop to reproductive maturity, indicating that these genes have effects outside of embryogenesis.
Most other diamonds show more evidence of multiple growth stages, which produce inclusions, flaws, and defect planes in the crystal lattice, all of which affect their hardness.
Significant recent progress in the analysis of aneuploidy in eukaryotes has revealed multiple novel features linked to aneuploidy such as growth defects, abnormal protein homeostasis and increased genomic instability.
Cells with telomere capping defects containing MRC1 or mrc1 AQ, a checkpoint defective allele, exhibit similar growth, suggesting growth defects of cdc13-1 mrc1Δ are not due to checkpoint defects.
The phenotypes of two of the mutants were analyzed in detail: one of the mutants that died showed defects in multiple organs (including the lung, heart and gastrointestinal tract), and one of the mutants that survived with growth defects also showed defects in the cerebral cortex.
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