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Sample size calculations—We used the NQUERY multiple group sample size programme for three groups.
We calculated our required sample size for α=0.05 and β=0.2 using the NQuery multiple group sample size programme.
We calculated the sample size based on an α value of 0.05 and β value of 0.2 using the NQuery program for multiple group sample sizes.
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The software is not designed to compare multiple groups of samples that arise from our experimental setting (23 samples in triplicate).
The software is not designed to compare multiple groups of samples that have arisen from our experimental setting (23 samples in triplicate).
We demonstrated that DFI is a highly effective and robust method for selecting gene features for RNA-seq experiments from multiple groups of samples.
For statistical analysis, one-way ANOVA was used for multiple group comparison across all samples in the experiment, followed by multiple testing correction to determine the false discovery rate (FDR; Benjamini and Hochberg method [ 38]).
Using the initial sample, the multiple group CFA (CFA-3 and CFA-5) included all 788, 544, 147 and 536 cases from acute care medicine, homecare, community care and ambulatory care, respectively.
When the Kruskal Wallis test was significant, we used Dunn's method to perform a nonparametric test of differences among multiple groups with unequal sample sizes and tied ranks (Elliott and Hynan 2011).
Based on the regression analysis on the test results of multiple groups of rock samples, Wang et al. (2007) obtained the change laws of rock compression coefficient based on the compression processes and concepts of rock compression coefficient.
In the current study, we used the definition of groups previously identified [ 6, 7, 11] mainly for the purpose of comparison, and it is possible that some groups we have designated as a single group might actually represent multiple groups because of sampling limitations.
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