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Our results provide evidence in favor of multiple genomic processes underlying the DMR methylation patterns 1 to 3 observed across UC tumors, and implicate pattern 1 DMRs as infrequent sites of RF binding, pattern 2 DMRs as frequent sites of CTCF/RAD21 as well as EZH2 binding, and pattern 3 DMRs as sites of frequent POLR2A occupancy.
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The data may also allow researchers to begin to identify sequence alterations occurring simultaneously in multiple genomic regions, which, when added together, drive phenotypic change — a process called polygenic adaptation14.
Reads mapping to multiple genomic positions were removed.
This generates diverse libraries of localized point mutations and can target multiple genomic locations simultaneously.
JMJD2 lysine demethylases (KDMs) participate in diverse genomic processes.
Maury, J. et al. EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in Saccharomyces cerevisiae.
Reads mapped to the multiple genomic positions were mapped randomly for WGS and discarded for dRNA and ssRNA-seq.
This is because some reads were counted multiple times when miRDeep2 assigned them to miRNA precursors, especially for miRNAs that map to multiple genomic loci.
Finally, exoglucanase was overexpressed in E. coli that carried multiple genomic copies of the celY gene.
The reads that matched to multiple genomic positions were discarded.
These results confirm the involvement of common variants in multiple genomic regions in regulating IOP and/or glaucoma risk.
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