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Additionally, repeat mutations are investigated in their native genomic environment and for multiple genomic loci (eventually genome wide) simultaneously.
Moreover, we find extensive gene redundancy and multiplicity, indicating transcription from multiple genomic loci.
WGA allows the analysis of multiple genomic loci from a single genome and has been performed on single cells from cell suspensions and from enzymatically-digested tissues.
Moreover, genome-wide screening studies identified multiple genomic loci in PGIA [ 20, 26- 29] that are syntenic with those described in RA [ 25].
DOI: http://dx.doi.org/10.7554/eLife.03703.005 Since the efficiency of cis-multiplexing with our CRISPR-Cas9/sgRNA system is nearly 100% using one sgRNA, we next tested its ability for trans-multiplexed CRISPR-Cas9 genome editing, that is introducing double-stranded DNA breaks at multiple genomic loci simultaneously, using tRNAs to drive the expression of the HDV-sgRNAs.
This is because some reads were counted multiple times when miRDeep2 assigned them to miRNA precursors, especially for miRNAs that map to multiple genomic loci.
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However, a fraction of the transcripts exhibited multiple-genomic loci.
During our analysis pipeline, there are many reads mapped back to multiple medaka genomic loci even if repeat-masked genome was used.
However, we still observed many reads with multiple mappable genomic loci (see Discussion).
Two additional genes, dysferlin and desmin, may also have genomic duplications, identified by multiple zebrafish genomic loci orthologous to the human protein sequences.
However, this analysis did display putative evidence for the loss of duplicate copies from past duplication events, inferred from the presence of multiple pseudogenized genomic loci.
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multiple genetic loci
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