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We simultaneously enriched DNA sequences flanking three different induced DSBs in the yeast S. cerevisiae as a model of multiple genomic hotspots.
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To investigate potential functions for S. pombe G4 motifs, we analyzed the distribution of the G4 motifs with respect to multiple genomic features, such as highly expressed genes, NDRs, meiotic DSB hotspots and so on.
However, recent work by Kruglyak and colleagues [ 68] in large, highly variable yeast populations using green fluorescent protein tags to quantify single-cell protein abundance has shown good correspondence between QTLs influencing mRNA and protein abundance; genomic hotspots were associated with variation in abundance of multiple proteins and modulating networks.
Reads mapping to multiple genomic positions were removed.
These loci behave as genomic hotspots for diversification because they underlie adaptive variation within and between species with both convergent and highly divergent wing patterns.
Structural genomic variants or copy number variants (CNVs) comprise submicroscopic deletions and duplications of chromosomal material, including both rearrangements at genomic hotspots as well as duplications and deletions with unique breakpoints.
The reads that matched to multiple genomic positions were discarded.
Two major genomic hotspots are on chromosomes 7 (7p11) and 12 (12q12-12).
Genomic hotspots and cool areas for introgression can be identified, as performed below, through the identification of distorted regions in which linkage disequilibrium (LD) is consistently strengthened through multiple generations.
This hotspot-targeted microarray consists of 135,000 probes (by Roche NimbleGen) with a median spacing 2.6 kbp in 107 genomic hotspots and 36 kbp in the genomic backbone.
The core action of LXR is focused on 112 genomic hotspots that contain 432 target genes.
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