To simplify and extract useful information, his group looked for combinations of histone marks that are highly recurrent at multiple genomic locations and that might track with particular functions in gene regulation.
When a particular grouping of domains within a polypeptide chain can perform a concerted function, that combination tends to reappear in multiple genomic contexts.
T. cruzi has multiple genomic loci with numerous tandemly repeated surface protein genes which may also function in immune evasion although this has been studied less extensively compared to T. brucei.
The potential function of these 54 proteins was examined by bioinformatic analysis using multiple genomic resources (UniProtKB/Swiss-Prot, Pfam, PROSITE and InterPro databases) (Table 2).
In perspective, by exploiting orthologous genes and multiple genomic sources, multi-species prediction can be applied to annotate poorly annotated species and discover new functions for uncharacterized genes in model organisms.
Thus, the combination of multiple genomic resources, including EST maps and FLcDNAs, will assist in finally revealing the complete structure and function of the barley genome.
In a previous study, we reported the use of the CRISPR/Cas9 system to simultaneously disrupt multiple genomic loci.
These methods are often supervised and allow the integration of multiple genomic data sources in different ways [2,3], thus generating reliable and robust results, often including the prediction of new protein functions [4].
To investigate potential functions for S. pombe G4 motifs, we analyzed the distribution of the G4 motifs with respect to multiple genomic features, such as highly expressed genes, NDRs, meiotic DSB hotspots and so on.
The reads that matched to multiple genomic positions were discarded.
