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From the 432 sequences obtained from the yeast assay, we manually curated them based on two criteria: first, we considered those sequences mapping within 20 kb of the transcriptional start of a gene or to the first intron, and second, we ruled out sequences that have been described as repetitive in multiple genome assemblies.
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Results: We evaluated the ability of multiple genome assembly programs to assemble bacterial genomes from a single, deep-coverage library.
Note that, in multiple genome assembly, nodes at a crossing point between two incoming and two outgoing edges are not necessarily repeats.
In multiple genome assembly, such nodes are not necessarily repeats since they can sometimes be shared between the genomes of two closely related species and represent orthologous sequences, conserved sequences (such as rRNA sequences) or horizontal transfer sequences.
In multiple genome assembly, such node is not necessarily a repeat since it is sometimes shared between the genomes of two closely related species and represents orthologous sequences, conserved sequences (such as rRNA sequences) or horizontal transfer sequences.
Here we examined the genomic architecture and repeat content of the 5S and 45S rDNA arrays in multiple human genome assemblies (including PacBio MHAP assembly) and ascertained contacts between the rDNA arrays and the rest of the genome using Hi-C datasets from two human cell lines (erythroleukemia K562 and lymphoblastoid cells).
In total six avian species (chicken, turkey, duck, zebra finch, ostrich and budgerigar) plus one lizard outgroup species (Carolina anole) were chosen for reconstruction of the ancestral karyotypes (for the reasons given in the sub-section Presentation of multiple avian genome assemblies).
In addition, these new features are also available in the Artemis Comparison Tool, ACT (Carver et al., 2005), which means datasets can be compared across multiple genomes or assemblies.
Multiple alignments of genome assemblies of six insects species to D. melanogaster (dm3) were obtained from UCSC Genome Bioinformatics Site (Kuhn et al. 2009) (http://genome.ucsc.edu).ucsc.edu
The current implementation of SNPchiMp was focused on integrating SNP information for cattle due to the number of assays currently available and multiple versions of genome assemblies.
Additionally, we have incorporated genome assembly and multiple genome alignment softwares into inGAP.
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