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Typing markers were found by multiple genome and gene comparisons, from which 23 canonical single nucleotide polymorphisms (canSNPs) and 11 canonical insertion-deletion mutations (canINDELs) were selected to provide phylogenetic guidelines for classification from genus to isolate level.
Sauropsid Kiss and KissR genes were predicted from multiple genome and transcriptome databases by TBLASTN.
Applying genetic and genomic methods to recent polyploids poses extra challenges due to the presence of multiple genome and gene copies; this can complicate short-read alignment and de novo assembly of genomes, but can also be useful in establishing patterns of relatedness among species and populations (Griffin et al. 2011).
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Chaining procedures are widely utilized to define genomic intervals that are consistently ordered and oriented in multiple genomes and are often labeled as syntenic (Bourque et al., 2004; Dewey, 2007; Dubchak et al., 2009; Kent et al., 2003; Paten et al., 2008; Pevzner and Tesler, 2003).
We predicted regulatory elements using three criteria: density of potential transcription factor binding sites (TFBS), sequence conservation across multiple genomes, and stretches of sequences with perfect conservation in human, mouse, and rat reference genomes.
It is worth noting that our miRNA filter depends on the folding of MCE cognates from multiple genomes and; therefore, is more stringent than the direct folding filter that we applied to confirmed miRNA, which relies on flanking sequence from one organism only.
If M << N, some clusters would contain reads from multiple genomes and precision would be low.
tasuke helps bioinformatics researchers of genome-wide resequencing projects to visualize a large amount of polymorphisms on multiple genomes and to release the data to the public.
With the completion of multiple genomes and increasingly good annotation, the primary sequence of almost any protein can be found in the databases.
Two types of motif identifying approaches were developed to detect motifs: one uses orthologous sequence alignments extracted from multiple genomes, and the other uses genome regions enriched with particular motifs in a single genome [ 3].
The outputs generated by different sequencing platforms are subjected to multiple analytical steps that usually start with sequence assembly or reference-based mapping and finish with simultaneous comparisons of multiple genomes and data visualisation.
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