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Successful transformation of rice employing Agrobacterium and recent advances in direct gene transfer by biolistics, evidenced by transfer of multiple genes, have removed some of the serious impediments in the area of gene engineering.
We discuss the observation that simultaneous perturbations of multiple genes have more pronounced effects, and present novel perspectives to use knowledge of growth regulatory networks to enhance crop yield in a targeted manner.
Indeed, during last years significant progresses in breeding for quality traits for food [110] and feed uses [79], as well as for resistances to biotic [91] and abiotic stresses [4] are being achieved, but several others, many of which are controlled quantitatively by multiple genes, have been more difficult to achieve.
First, for many diseases, multiple genes have been identified to collectively account for clinical phenotypes [7].
Multiple genes have been found involving in the pathogenesis of T2DM.
Screens of deletion mutations, called deficiencies (Dfs), each of which removes multiple genes, have also been used to find genes required for embryonic development.
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The use of RNA-guided Cas9 endonuclease for the concurrent engineering of multiple genes has been demonstrated in a number of plant species.
Furthermore, no evidence of recent segmental duplication involving multiple genes has been observed in sequenced plant genomes [ 36, 63- 65].
Co-regulation or co-ordination of the expression of multiple genes has been described in other arrangements and contexts.
With the rapid growth rate of newly sequenced genomes, species tree inference from multiple genes has become a basic bioinformatics task in comparative and evolutionary biology.
Multiple genes had functions in the metabolism of peptidoglycans (mureins), which form a mesh-like matrix within the periplasm and provide structural support to the cell wall.
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