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A large proportion of our empirical understanding of DDS comes from multiple generation selection experiments.
Solanum lycopersicum inbred Gh13 was derived from the TYLCV-resistant germplasm FAVI 9 [ 42] by multiple generation selection of single begomovirus-resistant plants in the field in Sanarate, Guatemala [ 41, 46].
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Behavioural differences were also noted in a multiple-generation selection experiment based on the performance of groups of half sibs, thus including direct and indirect genetic effects (Gunsett 2005).
However, the genomes of adult hybrids sampled from hybrid populations have already been subject to multiple generations of selection (at least 30; Rosenthal et al., 2003), making it difficult to evaluate the extent of selection on hybrids without genetic information.
Genetic variance is often maintained over multiple generations of selection, even though, in theory, inbreeding should reduce genetic variance [ 8].
We then compared survival, phenotypic variation and plasticity to determine whether hybridization affects adaptive potential after multiple generations of selection in the wild.
Constitutive expression of SAs from the chromosome did not affect growth and viability of the engineered E. coli strain, being stably maintained through multiple generations without selection pressure.
Older hatchery stocks that have experienced multiple generations of selection in a hatchery often have much lower fitness in the wild than more recently established stocks (Berejikian and Ford 2004; Araki et al. 2008).
Thus, studies using multiple generations of selection have repeatedly shown density to be a factor in evolution, not only determining the location of peaks, but also the steepness of surrounding adaptive landscapes.
We hypothesize that predictions based on ASE SNPs should be more accurate and persist longer over multiple generations of selection, than even whole genome sequencing containing the ASE SNPs as a subset.
To address the question of whether the hybridized populations possess more additive genetic variation directly, loci in coding regions of the genome require examination to determine whether genetic diversity at quantitative trait loci is actually still greater in hybridized populations after multiple generations of selection.
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