Owing to technical reasons, we were unable to use the pCAR-OF plasmid described above, as it would have involved multiple gene transfection into ALK+ALCL cells, which significantly decreased cell viability (not shown).
The osteogenic differentiation was compared by ALP activity (A) and mineralization (B) between different vector-mediated BMP2 gene transfections.
They have low cytotoxicity and have been widely used in biomedical science, such as gene transfections and drug delivery [24].
They have low cytotoxicity and have been widely used in biomedical science, such as gene transfections and drug delivery [34 36].
The in vitro gene transfections of two types of cells, HEK293 and NIH3T3, were investigated.
Nanoparticle design is critically important to address the multiple barriers involved in gene transfection; Table 5 summarizes the common strategies utilized in particle engineering.
Here, we take advantage of this property of peptide materials and design a DNA delivery vector equipped with multiple functions critical to efficient gene transfection.
Multiple cellular and molecular approaches were performed including gene transfection, siRNA transfection, RT-PCR, Western blotting, Rac activation assays and immunofluorescence.
Here, we show that while PBAE/DNA nanoparticles appear to use multiple pathways to enter cells, efficient cellular uptake and gene transfection depend on the cholesterol transport function of Niemann-Pick C1 (NPC1).
The present study furthers that effort by demonstrating culture and transfection parameters that permit suppression of multiple genes at once, resulting in a phenotypic outcome not achievable with single gene manipulations.
