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GeXP assays may be usefully applied in clinical and regulatory studies of multiple gene targets.
Design and construction of an optimal microbial cell factory typically requires overexpression, knockdown, and knockout of multiple gene targets.
To address this limitation, an isothermal reverse transcription-recombinase polymerase amplification (RT-RPA) assay based on multiple gene targets for specific detection of RRV was developed.
Identification of multiple gene targets that exhibit different modes of action toward a desired phenotype is a crucial step in strain improvement.
Downstream processing of these data has been hindered by the lack of robust multiplexed gene quantitative technologies facilitating study of the identified multiple gene targets.
We completed the system by engineering the respective plasmids harboring CRISPR/Cas9 and RecT for efficient curing such that multiple gene targets can be done iteratively and final strains will be free of plasmids.
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Multiple gene targeting has proved successful in applying the genome editing technologies in various model systems10,11,12,13.
Cao, J. et al. An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting.
This system permits custom-design options for relative quantification of multiple gene target expression, simultaneously in a single reaction, using nanogram quantities of total RNA template.
An integrated approach involving multiple gene targeting by applying the principles and knowledge of systems biology and bioinformatics would provide us with a holistic view and help derive some feasible solutions.
And most of all, how do miRNAs, with their multiple gene target modulation, really affect cellular functions?
More suggestions(15)
several gene targets
multiple gene interactions
multiple gene models
multiple gene transfections
multiple gene families
multiple gene signatures
multiple gene spots
multiple gene trees
multiple gene networks
multiple gene sets
multiple gene losses
multiple gene clusters
multiple gene phylogenies
multiple gene functions
multiple gene modules
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