In this study, we employed a combinatorial approach to interfere multiple gene functions of white spot syndrome virus (WSSV), the most lethal shrimp virus, using a single-batch of dsRNA, so-called "multi-WSSV dsRNA".
Multiple gene copies can represent hot spots for recombination and genomic rearrangements [ 70], in some cases leading to loss of symbiotic properties [ 71].
The BPI-like locus in mammals appears to have evolved rapidly through multiple gene duplication events, and is thus a hot spot for genome evolution.
Oligonucleotides spotted in multiple locations for use as potential controls included 76 Arabidopsis thaliana gene spots, 17 beta tubulin spots, 17 glyceraldehyde-3-phosphate dehydrogenase spots, 85 heat shock protein gene spots, 69 ribosomal protein gene spots, 112 randomly generated negative control spots and 470 blanks.
Human 43,000-spot microarrays (Stanford University) have 384 yeast gene spots used as negative controls.
were used to analyse the gene spots and process data.
The large size of COL4A3, COL4A4 and COL4A5 genes, comprising 48 53 exons each, and the lack of mutational hot spots make individual screening of multiple genes by Sanger sequencing difficult and expensive.
If multiple genes are regulated by a single trans-eSNP, the term eQTL hot-spot or trans-cluster is used (12, 13, 21, 28).
They traced this trait to multiple genes on five chromosomes.
We now know that multiple genes influence every phenotype.
