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Hereby we provide a framework to analyze the effect of multiple gene perturbation protocols, and their effect on cell differentiation processes.
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Following this synthetic biology strategy, herein we have computationally re-designed A. thaliana TRN by exhaustively exploring multiple gene perturbations in the form of gene knockouts or over-expressions.
Our work should enable the development of multiplex gene perturbation library screens for understanding complex cellular phenotypes.
Multiple measurements for one gene, perturbation and time interval are listed.
Furthermore, this FMB could be applied to interpreting different types of specific perturbations, including multiple gene knockouts and environmental stresses.
Complex diseases, such as diabetes often display multiple co-appearing clinical traits (phenotypes), which provide a better chance to determine whether a gene perturbation may cause such diseases than for simple genetic diseases consisting of only one or two phenotypes.
In Ingelsson lab, her main focus is identifying genes underlying insulin resistance using CRIPSR gene perturbation approaches.
With random gene perturbation, an SBN with perturbation can be constructed, as shown in Figure 8.
Without gene perturbation, our strains exhibit little embryonic lethality.
In particular, gene perturbation and intervention are considered in CSSBNs.
Also, the deletion of a single transcription factor may affect the expression of multiple genes, providing a global perturbation to the metabolite profile; a set of transcription factor mutants, therefore, is expected to contain a variety of different metabolite profiles.
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