Wang, Y. et al. A magnetic nanoparticle-based multiple-gene delivery system for transfection of porcine kidney cells.
Design of an efficient gene vector based on dendrimer remains a great challenge due to the presence of multiple barriers in gene delivery.
The extent of transgene expression largely depends on multiple aspects, such as gene delivery method, cellular physicochemical properties, and the traits of the construct [ 1].
This could be achieved using a different virus-mediated gene delivery system, multiple site injections, continuous infusion of larger volume using a cannula, and cell type-specific promoters.
This article will attempt to summarize the multiple attributes of this widely utilized gene delivery system.
Furthermore, massive AAV-mediated gene delivery achieved with multiple injections and different delivery routes was required either alone (28) or in combination with BMT (25) to ensure significant benefits, challenging the safety profile and the overall feasibility.
We have developed a mouse model for ovarian carcinoma by using an avian retroviral gene delivery technique for the introduction of multiple genes into somatic ovarian cells of adult mice.
However, during intracellular delivery of these carriers, multiple barriers have to be overcome or the efficiency of gene delivery will be impeded.
T cell-derived iN cells, generated by nonintegrating gene delivery, showed stereotypical neuronal morphologies and expressed multiple pan-neuronal markers, fired action potentials, and were able to form functional synapses.
In addition, the engineered nanoparticle has multiple applications in various biomedical fields, such as drug and gene delivery, biosensor actions, diagnosis of disease, and treatment of cancer.
