In contrast, miRNA drugs are developed with the idea of controlling multiple gene-components in the same or overlapping signaling-pathways.
In our earlier study (Acharya et al. 2012), RBf1 was found to bind multiple genes encoding components of conserved signaling pathways.
Indeed, multiple genes encoding components of the PI3K/AKT growth regulatory cascade have been causally implicated in human growth disorders (Supplementary Material, Fig. S1).
This was in line with zygotic activation of multiple genes encoding components of the key cellular organelles and structures: cell surface, ribosomes and microsomes with the greatest changes observed in the endoplasmic reticulum.
Our findings expand the phenotypic spectrum of the CDGs, demonstrating that multiple genes encoding components in this biologically crucial and complex pathway have the potential to harbour mutations that cause syndromes with symptoms largely restricted to defective neuromuscular transmission.
The "pattern specification process" category included 51 genes that were significantly up-regulated in at least one of the five experiments shown in Fig. 2 and Additional file 2. Amongst these genes (listed in Additional file 6) we find 13 homeobox genes and multiple genes encoding components of the Wnt, Shh and Notch signalling pathways (6, 4 and 3 respectively).
In agreement with this, our ChIP data identified in vivo Ttk69 binding in the vicinity of multiple genes encoding components of the Notch pathway (data not shown), indicating that Ttk69 has at least the potential to contribute to negative-feedback regulation directly.
Alternatively, extensive studies of miRNAs have revealed their importance in the regulation of multiple gene targets, including key epigenetic components.
Plants have clear homologs of the four core protein components of PRC2, often with multiple genes encoding each component [ 7].
The differences in DNA methylation between cell types involve multiple genes that are components of functional gene networks.
