Some predicted targets were likely to be targeted by multiple miRNAs at multiple targeting sites.
Compared to traditional drug delivery systems, nano-based drug delivery system have greater potential in a variety of areas, such as multiple targeting functionalization, in vivo imaging, combined drug delivery, extended circulation time, and systemic control release.
Multifunctional NPs have been designed to co-deliver multiple components, target the delivery of drugs by surface functionalization, and realize therapy and diagnosis simultaneously.
The targeting delivery to specific target or controlled release in targeted tissues can be realized through surface functionalization with targeting molecule.
This improved the efficiency of gold nanoparticle functionalization by targeting the subunits of the endotoxin.
Surface functionalization with hydroxy-PEG led to high colloidal stability and low untargeted cell binding, but still allowed covalent functionalization with targeting ligands.
Rosen et al. [ 35] provide a comprehensive review on the use of SPION, including their surface functionalization, for targeted cancer imaging and diagnosis.
This thesis aims to expand our current toolkit on functionalization to molecules that have multiple functionalities that can react with the surface.
Further functionalization with specific targeting molecules and stabilizing agent (e.g., PEG (poly ethylene glycol)) can result in fabrication of targeted nanotheranostic agent against lung cancer cells.
Incorporation of counterions, like spermidine, and surface functionalization with cell targeting or cell penetrating peptides have improved DNA loading and particle transfection.
