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"You have multiple fragments from the explosion, and people tend to have small pieces of metal and wood inside of them, and all of them have to be removed".
On the converse, experimental protocols using the culture of gut mucosa had become popular with the advent of endoscopy which allows explanting multiple fragments from the intestine.
Matt uses an initial rigid alignment of multiple fragments from the proteins.
Primers which either failed to amplify or amplified multiple fragments from any one of the 13 Musaceae entries were discarded.
> Because of the polyploidy nature of Brassica, SSR markers usually amplify multiple fragments from homologous DNA sequences, which could complicate or cause errors in the genotype scoring.
We next considered the overall complexity of the sequences in our database as our cloning strategy made it possible to obtain multiple fragments from the same cDNA.
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Amplification of inbred Ki11 RNA produced multiple fragments ranging from ∼1.2 kb to ∼2.1 kb in roots and from ∼1.2 kb to ∼1.6 kb in shoots.
At a lower annealing temperature (55 °C), amplification of single to multiple fragments ranging from approximately 100 4000 bp (fragments smaller than 100 bp were not counted) was observed in at least three taxa for each primer pair (Fig. 1).
In principle, multiple fragments derived from a single locus are expected to show the same phylogenetic relationship with homologs although confidence in phylogenetic inference tends to be low when the sequences are short.
For the 3'RACE, the gene specific-outer primer (4 hF) was located in exon 53, and the inner primer (3 hF) was located in exon 54 obtaining multiple fragments ranging from about 1 to 1.8 kb.
In rice, over 3000 of these gene-carrying transposon-derived elements were found in 440 Mb of sequence [ 19], and the acquisition of multiple gene fragments from multiple loci may result in the creation of new genes [ 20].
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