Sentence examples for multiple fragments and from inspiring English sources

The multiple fragments and linearized vector were assembled by ClonExpress™ II One Step Cloning Kit (Vazyme Biotech Co., Ltd., China) to generate plasmid pZ_P GAP -tapA.

Here we focus on the atomic structure of this elementary dimer, elucidated using X-ray crystallography on multiple fragments and electron paramagnetic resonance experiments on spin-labelled vimentin samples.

Thirty-five of these 394 proteins had experimental MWs available only in urine, ranging from 15 kDa to 82 kDa, of which 20 were within 20% variation of theoretical values, 3 were larger than 120%, and 11 were smaller than 80% of theoretical MWs, and one with multiple fragments and therefore omitted.

It was fortunate that the fracture of the phacoemulsification sleeve did not result in multiple fragments and therefore there was no retained foreign object in the present case.

Chromosomes in these bridges are usually prone to break into multiple fragments and often these fragments form micronuclei at the end of mitosis [ 14].

Pancreata were dissected into multiple fragments and fixed either for paraffin sections [zinc-buffered formalin (Polysciences), overnight] or frozen sections (4% paraformaldehyde/PBS; 4°C 2 4 hours), followed by processing as previously described (Kopinke and Murtaugh, 2010; Murtaugh et al., 2005).

This unusual length results from the incorporation in hobo elements of a considerable amount of extraneous DNA, including a number of partial transposon insertions from class 2 (such as multiple fragments of Chapaev3-1_AC) and class 1 (such as RTE Bov-B, CR1, and Sauria-SINE) (Kordis and Gubensek 1998; Piskurek et al. 2006; Shedlock 2006).

Sequences were assembled by multiple fragments from RACE and full length open reading frame was confirmed by PCR amplification and sequencing.

The term "shattered kidney" is used to describe a kidney ruptured in multiple fragments by severe and deep lacerations (Fig. 4a b).

We have now developed a protocol for cloning multiple fragments at once, and show here that at least 9 different fragments can be assembled together in a defined linear order and inserted into a recipient plasmid in one step, and that such procedure is so efficient that the majority (about 90%) of colonies growing on selection plates contain the desired constructs.

With NMDA treatment, tau is significantly degraded into multiple fragments (42K, 35K and 15K) including a dominant signal of calpain-mediated TauBDP-35K with minimal caspase-mediated TauBDP-45K.