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Multiple follicle development and ovulation have been attributed to higher elevations of FSH above the threshold level [ 19– 219.
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One limitation with most of the available FSH preparations is the relative short half-life in the circulation that dictates multiple daily injections for the desired follicle development and superovulation.
Therefore, our results suggest that differences in antral follicle numbers during the final stages of follicle development are not a key component of the mechanism underlying multiple ovulations in Trio carriers.
During follicle development, primordial follicles with Survivin depletion in the oocyte could develop into antral follicles with normal granulosa and cumulus cells, and release eggs.
Another interesting category is hair follicle development, which also showed a higher level of population differentiation (GO: 0001942, λ = 4.09, P= 2.07E-08 after multiple testing).
Several candidate genes for multiple ovulation were identified by combining microarray analysis of restricted vs. ad libitum feeding, literature searches and QPCR expression profiling throughout follicle development.
A multiple thyroid follicle model was used to calculate the contribution from surrounding layers of follicles to the follicle cell nuclei in a centrally placed follicle, based on the previously published multiple follicle model [[19]].
The multiple follicle thyroid model used in this work is a simplification, with a single spherical layer of follicle cells surrounding the central follicle and the neighbouring follicle lumen simulated as a spherical shell around the outer cell layer.
This biomimetic configuration greatly facilitates follicle development to antral stage.
Fat supplementation had little effect on follicle development.
For radioiodine homogeneously distributed in the follicle lumens our calculations for the human multiple follicle models gave up to 90% lower mean absorbed dose compared with MIRD formalism.
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