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This most probaby reflects the fact that a single dextramer carries multiple fluorochrome molecules.
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In Figure 1, the fluorochrome molecules bound to the Aβ peptides are excited by blue laser irradiation (λex) at a high energy level and emit visible fluorescent light (λem) at a low energy level.
ProSense 680 is a composite probe that is based on a poly-lysine chain on which a number of fluorochrome molecules were linked on the side chain of the lysine moiety, as well as a number of MPEG molecules.
Individual dextramers conjugated to PE, APC and fluorescein isothiocyanate (FITC) averaged 3, 7 and 33 fluorochrome molecules and typically, 6, 3 and 13 streptavidins with the typical number of streptavidins per dextramer exhibiting the expected Gaussian distribution.
Flow cytometry is a system based on the principle of light scattering, light excitation and emission of fluorochrome molecules to generate specific multi-parameter data from particles and cells simultaneously.
While stained with multiple fluorochromes, the C. vulgaris cell wall consisted of β-polysaccharides could be clearly observed (Additional file 1: Figure S5 and Figure S6) [Note: cellulose belongs to β-polysaccharides].
These multiple repeats enable a single FnBPA molecule to bind multiple fibronectin molecules [25] [27].
Consumption of multiple SAM molecules per small molecule product for radical SAM-based biochemistry has been reported [33], [34].
Multiple adhesion molecules and their regulators are expressed at different levels between hESC and phESC derivates.
It is understood to co-ordinate and bind multiple water molecules.
So there may be multiple signal molecules involved.
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