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Using multiple fluorescent strains of M. marinum, it was found that superinfecting mycobacteria readily home to existing granulomas, indicating that this structure could dynamically rearrange to accommodate incoming macrophages and their phagocytosed mycobacteria (Cosma et al., 2004).
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A (4-piperidinylfluorophenyl) imidazo1,2-a]pyridine was applied to a multiple fluorescent chemosensor [13].
Multiple spectrally modified mutant versions and novel fluorescent proteins from other species have subsequently been reported and used for labelling cells with multiple fluorescent protein fusions.
However, few methods to obtain multiple fluorescent colors for various cell types have been developed.
Multiple fluorescent proteins are also simultaneously used to monitor different aspects of biological systems.
Because we only inoculated plants with fluorescent strains, the ratio of fluorescent to non-fluorescent bacteria was also recorded.
Insertion of epitope tags into fluorescent proteins is highly useful for immunological detections of multiple fluorescent proteins.
Using fluorescent microscopy to visualise the fluorescent strains confirmed that only a proportion of the bacilli expressed the fluorescent reporter (Figure 4).
For fluorescent strains a saturating level of 100 μM IPTG was used to induce sfGFP expression.
High resolution and rapid simultaneous acquisition of multiple fluorescent wavelengths can be achieved by utilizing concurrent imaging with multiple detectors.
Fluorescein angiography showed bilateral hypofluorescence in the fovea, surrounding hyperfluorescence, and multiple fluorescent leakages.
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