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Although a single exponential decay model requires the fewest photons to fit reliably, it is desirable to employ more complex decay models because the fluorescence from skin originates from multiple fluorescent species, each of which are themselves likely to exhibit complex decay profiles.
All of the determined average spectra center of mass ranged at less than 4.8nm apart, inferring spectral phasor is able to spectrally separate multiple fluorescent species below 5nm.
Asymmetric spectral profiles (eccentricity ≥ 0.66) of the major spectral peaks in designs C3 4, C4 3, C4 4, and C7 7 might be due to the coexistence of multiple fluorescent species with very close emission peaks.
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In fact, multiple fluorescent proteins have been isolated from a single cnidarian species [16], [17], [18], raising the following questions: How many and what kind of GFP-like genes are encoded by the Nematostella genome?
Multiple spectrally modified mutant versions and novel fluorescent proteins from other species have subsequently been reported and used for labelling cells with multiple fluorescent protein fusions.
Longer wavelength fluorescent species have been assigned to hydroxylation of the aromatic rings of PET.
A (4-piperidinylfluorophenyl) imidazo1,2-a]pyridine was applied to a multiple fluorescent chemosensor [13].
However, few methods to obtain multiple fluorescent colors for various cell types have been developed.
Multiple fluorescent proteins are also simultaneously used to monitor different aspects of biological systems.
I t) is well fitted by single or multiple exponentials
Insertion of epitope tags into fluorescent proteins is highly useful for immunological detections of multiple fluorescent proteins.
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