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Multiple fluorescent proteins are also simultaneously used to monitor different aspects of biological systems.
Moreover, sophisticated biosensors engineered to contain single or multiple fluorescent proteins, including FRET-based biosensors, spatiotemporally unveil molecular mechanisms underlying physiological processes.
One way scientists can observe and quantify processes in living cells is to engineer the genomes of animals to express multiple fluorescent proteins and then quantify those signals by various imaging techniques.
Insertion of epitope tags into fluorescent proteins is highly useful for immunological detections of multiple fluorescent proteins.
Our method allows the detection of small spectral variations in depth in the living brain, which is essential for imaging efficiently transgenic animals expressing combination of multiple fluorescent proteins.
In fact, multiple fluorescent proteins have been isolated from a single cnidarian species [16], [17], [18], raising the following questions: How many and what kind of GFP-like genes are encoded by the Nematostella genome?
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Multiple spectrally modified mutant versions and novel fluorescent proteins from other species have subsequently been reported and used for labelling cells with multiple fluorescent protein fusions.
The development of spectral variants allows the study of multiple fluorescent protein fusions at the same time in a single cell [3], [4].
The L52 linker consisting of an elastic GPGGA repeat (Grashoff et al., 2010) showed improved response to ABA in combination with multiple fluorescent protein pair combinations.
Although image deconvolution can be successful in improving image detail and contrast, as well as in reducing background haze, 21 it is unclear whether this method is always appropriate for quantitative spatial characterization in images containing same-color multiple fluorescent protein copies (>2 copies).
By crossing Actb-MBS and MCP mice, a double homozygous mouse line, MCP × MBS, was established to visualize endogenous β-actin mRNA labeled with multiple green fluorescent proteins.
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multiple soluble proteins
multiple fluorescent probes
multiple fluorescent channels
multiple fluorescent parameters
multiple fluorescent images
multiple metazoan proteins
multiple fluorescent markers
multiple different proteins
multiple fluorescent immunostainings
multiple associated proteins
multiple functional proteins
multiple fluorescent colors
multiple fluorescent strains
multiple fluorescent dyes
multiple fluorescent knock-ins
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