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The Lecia confocal microscope was used for observing multiple fluorescent probes (Cy3 and Cy5 channels) and tracking the colocalization of fluorescence within the cells.
As a result, the SHG signal can be distinguished from the fluorescence, and, in essence, the collagen can be imaged independently when even multiple fluorescent probes are used.
Researchers can now use multiple fluorescent probes to quantify effects on intracellular molecular events, measure phenotypic changes, and provide contextual information about cellular pathways not discernible by traditional single‐parameter, end point experiments.
Multiple fluorescent probes, rapid image capture, and immunofluorescence under nonfixation conditions allow for measurements of the location and intensity changes of endogenous proteins upon addition of ATP or upon addition of other proteins or regulatory factors.
In fluorescence microscopy of eukaryotic cells, automated single-cell quantification can be achieved using multiple fluorescent probes and channels in a single experiment.
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An application of the microsphere feeding assay that was not explored in this study is the use of multiple fluorescents probes.
Using multiphoton fluorescence correlation spectroscopy (MP-FCS), we recently reported multiple diffusive mechanisms of fluorescent probes in dextran (multiple microviscosities experienced by the probe, analogous to multiple discrete diffusion coefficients) and proposed that the probe can be used as a reporter of nanostructuring of the environment (4).
The next-generation molecular probes fusing multiple fluorescent dyes, drugs, and multiple NPs into a single nanoprobe should provide superior fluorescence, appreciable sense capabilities, enhanced MRI contrast, and targeted drug-delivery capabilities.
By introducing multiple hydroxyl groups to the fluorescent probes (perylene diimide derivatives) which builds up hydrogen bonds between the probe and polymer matrix, the dynamics is discovered to be retarded.
HCS (Cellomics Inc, Pittsburgh, PA, USA) enables concurrent quantitative measurement of multiple independent cellular phenotypes using fluorescent probes.
We used genetically encoded unnatural amino acids and bioorthogonal labeling reactions to engineer multiple fluorescent donor-acceptor pairs to probe ligand-directed structural changes in GhrR.
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