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Rafts are now defined to have diameters of just 10 200 nm (Keystone Symposium on Lipid Rafts and Cell Function; [19]), but published reports of confocal imaging studies using fluorescent raft components [69] report sizes (∼1 µm) similar to those found here and likely reflect amplification of apparent size by attachment of multiple fluorescent molecules and/or aggregation of smaller raft domains.
This results in fewer, brighter independent diffusers, each with multiple fluorescent molecules.
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While multi-channel fluorescence microscopy overcomes the spatial resolution issues of light microscopy [ 37], the approach is limited to the visualization of the particles relative to one or a few intracellular components, which in addition are studied under artificial conditions in the presence of multiple fluorescent marker molecules.
These concentrated and enhanced electromagnetic fields can excite fluorescent molecules with highly improved fluorescence efficiency.
However, the ability to observe FRET depends on multiple parameters, including the proper spatial orientation of the fluorescent molecules, which is hard to predict even for known interaction partners.
So that the fluorescence spectra of the static quenched fluorescent molecules change.
After collision, the fluorescent molecules return to the ground state, so that the fluorescence spectra of the dynamic quenched fluorescent molecules do not change.
The fluorescence density of each frame was very high, and the fluorescent molecules were overlapped.
Other signals come from naturally fluorescent molecules within the cells.
Most fluorescent molecules stop working within a few minutes at room temperatures.
The color reproduction of the diodes was also improved after the Kodak laboratories found a way to introduce fluorescent molecules into the layers of organic molecules.
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