Sentence examples for multiple fluorescent markers from inspiring English sources

We have demonstrated a practical way to follow multiple fluorescent markers, and construct complete cell pedigrees over many cycles of division and growth comparable to conditions in liquid culture, combined with the ability to pulse with inducers.

Unlike imaging one biomarker at a time, the real-time simultaneous imaging of multiple processes using multiple fluorescent markers inevitably faces additional challenges of avoiding interference effects as each fluorescent marker is now exposed not only to the light excitation intended for it but also the excitation wavelengths necessary for other markers.

Data analysis was performed with FlowJo Software (Tree Star, Version 8.6.6 from FlowJo Africa, http://www.flowjo.com/home/africa/) with Boolean gating to analyse cells with multiple fluorescent markers.

In contrast, when multiple fluorescent markers are used for staining, stained specimens cannot be stored for a long time due to the poor stability of fluorescent dyes.

This indicates that tissue sections can be stored at room temperature for a relatively long time after they are stained with multiple fluorescent markers, which could open a door for pathological diagnostics.

To date, the only reporters that achieve this without the need to sequence breakpoints are traffic light reporter (TLR) systems that elegantly combine multiple fluorescent marker genes to detect mutNHEJ and GT (25) or mutNHEJ, GT and SSA (26).

While multi-channel fluorescence microscopy overcomes the spatial resolution issues of light microscopy [ 37], the approach is limited to the visualization of the particles relative to one or a few intracellular components, which in addition are studied under artificial conditions in the presence of multiple fluorescent marker molecules.

Imaging single cells with fluorescent markers over multiple cell cycles is a powerful tool for unraveling the mechanism and dynamics of the cell cycle.

We have combined this device with IMSTAR Pathfinder™ automated image capture and image analysis system to conduct high resolution image-based phenotypic screening on multiple parameters obtained using three fluorescent markers [5].

Therefore, detection of cytopathological markers simultaneously using multiple fluorescent dyes would be valuable in the pathological diagnosis to differentiate renal tumors and cancer subtypes.

The DNA hybridization to complementary DNA targets labelled with fluorescent markers was confirmed using fluorescence microscopy.