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In addition, multiple fluorescent channels can be recorded simultaneously, which offers the possibility to directly observe the development of individual biofilm components [ 14].
Seeded cells could be subsequently stained with vital dyes or specific probes and imaged on multiple fluorescent channels with sufficient resolution to detect subcellular distributions.
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In fluorescence microscopy of eukaryotic cells, automated single-cell quantification can be achieved using multiple fluorescent probes and channels in a single experiment.
The Lecia confocal microscope was used for observing multiple fluorescent probes (Cy3 and Cy5 channels) and tracking the colocalization of fluorescence within the cells.
The system includes three fluorescent channels and brightfield to simplify fluorescence imaging for cell culture applications.
A (4-piperidinylfluorophenyl) imidazo1,2-a]pyridine was applied to a multiple fluorescent chemosensor [13].
Dense fields of cells can be segmented from a phase image, leaving the fluorescent channels free.
Insertion of epitope tags into fluorescent proteins is highly useful for immunological detections of multiple fluorescent proteins.
The crosstalk between the two fluorescent channels was below 0.1%.
lsm files to extract mean cell intensity in all 4 fluorescent channels.
Maximum intensity projection was made for fluorescent channels of confocal scans.
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