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Multiple flies were detected and tracked simultaneously using an array of calibrated and synchronized digital video cameras operating at 60 frames per second, allowing for real-time analysis of fly movement.
For D. affinis, multiple flies were placed in each vial, as the flies appear more likely to lay eggs when maintained at a higher stocking density.
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Tracking of gene expression using DsRED reporter transgenes in multiple flies was accomplished using published methods [33].
Any area with multiple drain flies is likely a problem.
Flies were separated by sex within three days of eclosion to ensure virginity and were kept in sex-specific groups of 5 to 10 in fresh food vials until sexually mature, 21-28 days post-eclosion (except for multiple-choice experiments, where flies were kept in groups of 30-35 individuals).
To test this hypothesis and to identify modifiers of the sucrose intolerance phenotype, we screened knockdowns of the selected genes (Table 1) using the now-classic GAL4/UAS system [ 23]. Fly lines containing inducible RNA-interference (UAS-RNAi) elements were acquired for most of these genes; multiple fly lines were available for many loci, and a total of 137 RNAi fly lines were tested.
Flies were exposed to multiple looming stimuli in an enclosed arena to increase the likelihood of seeing both escape and freezing responses.
Flies were likely to be heteroplasmic for that particular region as if multiple recombinants are carried in this line.
Tsetse flies were eradicated.
Flies were tested and imaging results are displayed as described in Figure 5. Average changes in fluorescence ±s.e.m. of multiple flies.
Canonical LTM in flies is induced by multiple training with rest intervals, and is mediated by a transcription factor, CREB and its binding protein, CBP.
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