Temperature of the slide should be controlled (+37°C) by using a stage warmer on a phase-contrast microscope, the depth of suspension on the slide should be standardized and multiple fields near the centre of the slide examined.
In this study, we extracted 32 SCZ drugs based on the records in multiple fields from the DrugBank database and the suggestions from one of us (AHF), a practicing psychiatrist treating mostly psychotic disorders.
The microscope allows the acquisition of multiple fields on the X, Y and Z axes and is computer assisted.
This research work brings true added value to multiple fields in the scientific domain; from the performance analysis of hospital care [ 17, 18] to the on-going exploration of pharmaceutical trials data [ 19], among others [ 20].
In a few cases, fields were visited for direct collection of freshly harvested seeds; while farmers did not usually mix varieties in the same field, they often harvested multiple fields at the same time and could identify each variety based on the seed and panicle appearance (Supplementary Fig. 1).
In those cases the search can be refined by combining multiple fields from the database – i.e. Taxon, Organism Name, Protein Name (Recommended Name, Alternative Name and Submitted Name) and the Gene Ontology Terms for Molecular Function, Biological Process and Cellular Component.
A search through the database can be performed separately on a particular feature in the simple search as well as simultaneously on multiple fields using the advanced search option.
The unbiased inspection at time 0 (90 min post plating) of multiple fields revealed the acquisition of GFP-H-RasG12V-labeled membrane patches in a significant percentage of the red-labeled Jurkat cells.
The paper is certainly long; however, I would like it to be able to serve as a reference for future works, as well as show the breadth of evidence supporting the ideas presented here, in order to draw investigators from multiple fields into the discussion.
