Based on their close proximity to the basement membrane and on post-irradiation kinetics, AGAT-1 + cells likely represent a relatively stable transition at which multiple fate decisions could be made.
Such similarities, as well as clear phenotypic differences (data not shown), suggest this level of complexity allows the DIF signal to be integrated into multiple fate choices during development.
Gilbert, N., Lutz, S., Morrish, T. A. & Moran, J. V. Multiple fates of L1 retrotransposition intermediates in cultured human cells.
Nixon, R. A. & Logvinenko, K. B. Multiple fates of newly synthesized neurofilament proteins: evidence for a stationary neurofilament network distributed nonuniformly along axons of retinal ganglion cell neurons.
First, low level co-expression of Scl, Mesp1 and Tbx6 (required to specify blood, cardiac and paraxial lineages, respectively) confirms multi-lineage priming and suggests that progenitors can potentially adopt multiple fates.
However, due to the complexity of chemical and mechanical cues specifying differentiation outcomes, development of arbitrarily complex geometric and structural arrangements of cells, adopting multiple fates from the same initial stem cell population, has been difficult.
The capability to engineer microenvironmental cues to direct a stem cell population toward multiple fates, simultaneously, in spatially defined regions is important for understanding the maintenance and repair of multi-tissue units.
Human memory CD4+ T cells acquire multiple fates when responding to infection or vaccination.
We also found that naïve CD8 T cells can adopt multiple fates under a variety of conditions and that the timing of activation during infection is an important factor.
To address the resulting uncertainty about the significance, or even existence, of LSCs, we used multiple fate-tracing strategies in mice with CDE-diet-induced chronic liver injury, all of which failed to provide convincing evidence for BECs or mesenchymal liver cells giving rise to new hepatocytes.
Our observations showed that once a nascent branch forms, it can have multiple fates.
