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The second goal concerned the impact of multiple familiarisation trials.
It was found that after multiple familiarisation trials, objects could now successfully be recognised as familiar by rats with perirhinal cortex lesions, both following a 90-min delay (the longest delay tested) and when object recognition was tested in the dark after familiarisation trials in the light.
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This possibility led us to determine whether rats with perirhinal cortex lesions can overcome their preferential exploration deficits when given multiple object familiarisation trials prior to that same (familiar) object being paired with a novel object.
This group was then given 20 familiarisation trials locating food with the use of a mirror (Medina et al. unpublished data).
Familiarisation trials were not provided to minimise fatigue and time required to administer the test.
Participants will complete two submaximal familiarisation trials followed by 3 4 Maximum Voluntary Contraction (MVC) trials during which they will be asked to perform maximal effort for 3 4 s.
An initial set of 12 familiarisation trials will be completed at the start of each laboratory session in order to dampen within-session habituation effects [ 47].
This finding suggests that the disruptive effects of PRh lesions on object recognition may be confined to when the familiar object has only had a limited number of pre-exposure (familiarisation) trials.
These latter findings reveal: (i) the presumed recruitment of other regions to solve recognition memory problems in the absence of perirhinal cortex tissue; and (ii) that these additional recognition mechanisms require more familiarisation trials than perirhinal-based recognition mechanisms.
Determining the required number of familiarisation trials was more systematically tested in Experiment 2. Experiment 2b also examined whether familiarity learning by rats with PRh lesions is qualitatively different to that in normal rats, i.e. whether the spared recognition processes have different characteristics.
Experiment 2 then showed that these rats required only one, or at most two, extra familiarisation trials to reach control levels on this measure, and that the available recognition information included non-visual cues as the animals could transfer from a sample in the light to a recognition test in the dark.
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