Only CDH12 and PMP22 showed a difference between multiple expression studies and our results.
The predicted upregulated mediators were consistent across multiple expression thresholds and included TNF-α, TGF-β, IL-1β, NFκB, and the SWI/SNF component Smarca4/Brg1.
A recent detailed review describes the methods applied to obtain strains containing multiple expression cassettes and provides a summary of published data showing correlations between copy number and expression levels of intracellular as well as secreted proteins.
The HapMap lymphoblastoid cell line data consist of multiple expression datasets and cover several continental-level populations.
Although GEO2R Analysis allows users to compare multiple expression data and then identify DEGs, GEO Profiles can only display the expression level of one gene across different samples in each dataset.
It also contains a well-curated and annotated genome browser complete with Mu and Ac/Ds insertional mutations (see below) and multiple expression analysis tools.
We used a high-throughput cloning approach using the Gateway system and liquid-handling robots for cloning the 22 ORFs into multiple expression vectors, expression screening, and activity screening.
To assess the relationship between age at draw and multiple expression levels, we employed the LASSO algorithm of Tibshirani (Tibshirani, 1996; Efron et al., 2004) to build a linear model of age at draw as a function of multiple expression levels.
In this method, however, thousands of expression profiles are related with sequence polymorphisms across the genome through their correlated variations, which results in a large number of mappings that make it difficult to consider simultaneously the relationships between multiple genomic regions and multiple expression profiles.
Multiple expression datasets from many brain and peripheral tissues of inbred and RI strains were used in this analysis (see Text S1 for additional methods).
