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Multiple experiments demonstrated that unlike wild-type cells, constitutively active Stat-5A1*6-transfected cells were only minimally affected, with an average of only 6 10% of the transfected cells succumbing to a tumor-supernatant-induced apoptotic death and theaflavins were able to protect them further (Fig. 4B).
Multiple experiments demonstrated that although control T cells were highly sensitive to tumor supernatant, with an average of 42% of Jurkat cells testing positive for apoptosis, Bcl-2-transfected cells were only minimally affected, with an average of only 12% of the transfected cells succumbing to a tumor supernatant-induced apoptotic death (Fig. 5B).
Multiple experiments demonstrated 78% reduction in DNA synthesis during the first day after exposure to 20 micrograms ml-1 HpD plus 1140 Jm-2 light followed by progressive recovery to the normal rate after 4-6 days.
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The quantitative data from multiple experiments demonstrate that the Myo Va motor domain (41-505) significantlyicantly more binding activity compared to the 800-881 construct (Fig. 2D, p<0.015).
Furthermore, the % methylation did not change with differing amounts of template DNA or between multiple experiments, demonstrating high efficiency and reproducibility of the MethyLight assay.
Careful experiments demonstrated multiple deviations from the "rational" approach of the standard Utility model [ 2], and new models were proposed that attempted to explain many of these observed decision behaviors.
Together, these experiments demonstrated that reversible thiol protection schemes provide a rapid, straightforward method for producing multiple, site-specific modifications.
These experiments demonstrated that this LRET assay can easily be applied to PPInts with recombinant proteins and that multiple emission peaks of the lanthanide ion can be exploited for "multicolor" LRET.
Our initial experiments demonstrated that IRF6 localized to the cytoplasm and, consequently, was unable to activate a luciferase reporter containing multiple copies of its consensus binding site.
Previous experiments demonstrated systemic RNAi in ticks after injecting dsRNA into unfed ticks, and the resulting gene silencing could be detected in multiple tick tissues [ 14].
Evidence for a role of Wnt proteins in hematopoiesis arose from experiments demonstrating that multiple Wnts could expand hematopoietic stem/progenitor cells in culture [ 31].
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CEO of Professional Science Editing for Scientists @ prosciediting.com