In order to compare multiple mouse lines, experimental procedures were standardized as described in detail in Figure S1.
We also applied location and scale adjustments to the normalized read counts to remove technical variation among multiple batches (Supplemental Experimental Procedures).
This short movie (30 s) was presented repeatedly over a 30 min period, enabling us to identify many units (∼30% of all cells) with highly reproducible firing patterns across multiple presentations (see Supplemental Experimental Procedures, Natural Movie Correlation Analyses).
The proposed approach detects consistently coexpressed PPI modules across multiple datasets (for details see Experimental procedures and Data S1, Supporting information).
Extended Experimental Procedures Bioinformatics Multiple sequence alignments were created by using the L-INS-I algorithm of the MAFFT package (Katoh et al., 2002 ).
Results shown are representative of multiple trials, odorants, and concentrations; experimental procedures were adapted from those described previously (Waggener and Coppola, 2007 ).
In Figure S3 and the Extended Experimental Procedures, we present multiple lines of evidence that fusion occurs preferentially in obligate complexes, including a lower tendency for fusing subunits to be observed in isolation and a much higher propensity for correlated messenger RNA (mRNA) expression.
This alignability shows a marked increase at approximately 11 13 species, reflecting the contribution to the multiple alignments of the ten highest-quality genomes (Experimental Procedures).
We reasoned that a single-plasmid based approach would simplify the experimental procedures by eliminating the need for multiple transformations and more than one resistance marker.
Psycon supports experimental procedures in which the stimulus is presented in multiple intervals, either "standard/reference" or "odd-ball/variable," in a random order.
