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We combined the generation of 3D functional tissue units with the fine control over the local microenvironment (e.g. oxygen gradients), and developed an effective strategy to enable the high-throughput screening of multiple experimental parameters.
Here, we describe a human cell-based screening assay, optimized by varying multiple experimental parameters that resulted in an assay that was used to demonstrate full and potent neutralization by the parental, chimeric and humanized antibodies.
Thus, one of the objectives of this study is to examine multiple experimental parameters to provide biologists a framework for using synchrotron phase-contrast imaging.
For comparison between multiple experimental parameters two-way analysis of variance (ANOVA) was used followed by Bonferroni post hoc test in GraphPad Prism 5 (GraphPad, La Jolla, CA, USA).
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Bubbles could also experience multiple collisions with the wall at certain experimental parameters (geometry of a channel, range of superficial liquid velocity, bubble size, etc).
The model was evaluated on primary human hepatocytes and parameter identifiability analysis was performed under multiple experimental constraints.
We are able to portray a robust design that encompasses multiple measurement modalities, along with the ability to change many experimental parameters with ease.
In another study including multiple laboratories, Richter and colleagues tried to investigate whether strict standardization of experimental parameters beneficially affects reproducibility of mouse behaviour [ 17].
Importantly, with a single set of parameter values, the resulting model agrees well with multiple experimental measurements of both single cells and populations of cells from several laboratories.
The protocols are most useful for complicated assays like the Haplotype-specific Chromatin Immunoprecipitation, where multiple molecular biology techniques has to be carried out and the fine-tuning of experimental parameters vital to eventual success.
Furthermore experimental parameters such as instrument response function and chirp can be extracted and the interaction of populations with multiple (Gaussian shaped) pulses can be modelled.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com