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For a given GO annotation, multiple evidence passages may appear in the paper, some being more specific with experimental information while others may be more succinct about the gene function.
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The passages that indicated GO evidence were annotated as evidence passages, and for each evidence passage GO terms and associated Entrez Gene IDs were annotated.
Manually curated GO evidence passages were used as the gold standard for evaluating team submissions.
The NSCs co-cultured with Cd-MECs retained self-renewal capacity, as evidenced by multiple passages (> 3) of structures formed in Matrigel.
This holds true in cell-free conversion assays [ 6, 13, 93], cell assays [ 21, 48] and bioassays using transgenic or wild-type animals [ 31, 47] as evidenced by sustained PrPC to PrPTSE conversion and/or replication of infectivity in multiple serial passages in which the original seeds were gradually diluted out.
The evidence for a GO annotation could also be derived from multiple lines of experimentation, leading to multiple text passages in a paper supporting the same annotation.
Multiple Bible passages support this.
However, such a difference could not be detected after multiple cell passages.
The mRNA of DLD1-derived aneuploids was isolated from multiple different passages [ 13].
In addition, the analysis of multiple orthoxenograft passages revealed that this highly altered genome remained stable along successive xenograft passages (Supplementary Fig S4A and B).
Under this experimental setting, we passaged the HTR8/SVneo spheroids multiple times (5 passages and continuing) without their loss of spheroid-forming abilities.
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