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Remarkably, we also found that 13 out of the 24 mutations appear to be fast-acting, making them particularly useful for careful dissection of multiple essential requirements.
With conditional alleles, multiple essential requirements can be examined by shifting at different times from the permissive temperature of 15°C to the restrictive temperature of 26°C.
Furthermore, one gene can have multiple essential requirements during an organism life cycle, precluding investigation of all but the first essential requirement in progeny homozygous for a non-conditional mutation.
Particularly with fast-acting TS mutations, one can identify multiple essential requirements, and define temperature-sensitive periods of gene requirements, sometimes even within a single cell cycle, or during the entire life span of an organism, by performing temperature up-shifts and down-shifts at different times [8], [9].
Similar(56)
We obviously have lots of standards and multiple sourcing requirements".
The DaO imposes multiple reporting requirements.
Here, the Panasonic performed well across multiple requirements.
When available, fast-acting temperature-sensitive (TS) gene mutations are perhaps the most powerful tool for dissecting multiple requirements for essential genes.
The approach is complex due to its multiple camera requirement.
Multiple functions and essential to Ayurveda".
Multiple voices are essential in the selecting of these sites.
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