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As new strategies to construct and manage multiple enzymes in ever more complex systems are developed, novel applications emerge.
In addition, the tailor-made pore structures of MOFs realized the fitting of multiple enzymes in position for highly efficient enzyme cascade (Wu et al. 2015a, b; Lian et al. 2016).
In addition to engineering, this work better defines the macrocyclization mechanism, provides the first biochemical demonstration of Ser/Thr posttranslational prenylation, and is the first example of reconstitution of a native, multistep RiPP pathway with multiple enzymes in one pot.
Multifunctional enzymes comprising the direct surface display of multiple enzymes in a non-complex form are very high-molecular weight proteins of one or several CBMs and two or more CDs for effective substrate targeting and efficient degradation of plant cell walls, respectively (Moraïs et al. 2012; Smith et al. 2015).
The cellulosome architecture (Fig. 4) is dictated by a primary scaffoldin subunit, consisting of repeating units of cohesin (type I) modules that engage in high specificity and or affinity protein protein interaction (K D < 10−9 M) with type I dockerin-containing enzyme, allowing the assembly of multiple enzymes in a spatially defined manner (Park et al. 2014; Stern et al. 2015).
This type of regulation often involves allosteric regulation of the activities of multiple enzymes in the pathway.
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In particular we find that multiple enzymes involved in ATP generation are hyper-acetylated in skeletal muscle mitochondria, for many of which it is known that the enzymatic activity of their bacterial orthologs is regulated by lysine acetylation (Wang et al., 2010).
C4 metabolism is generated by the increased abundance and M or BS-specific expression of multiple enzymes (shown in purple), which are expressed in both M and BS cells of C3 leaves.
There are multiple enzymes involved in the production of O2●- and its derivatives in the vasculature, in particular, vascular protein kinase C (PKC -activated NAD(PKC -activated4].
This study also shows that the ESBL phenotype spread in this hospital is due to multiple enzymes found in different genera of bacteria.
Work carried out in Arabidopsis has shown that there are multiple enzymes involved in the synthesis of the glucuronoxylan backbone (IRX9, IRX10 and IRX14), substitution of the backbone (GUX1/GUX2) and synthesis of the reducing end oligosaccharide (IRX8, FRA8 and PARVUS) [ 30, 31, 40].
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Justyna Jupowicz-Kozak
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