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Because of the ability to control multiple, if not all steps in a particular metabolic pathway, TFs provide attractive tools for overcoming flux bottlenecks involving multiple enzymatic steps, or for deploying pathway genes in specific organs, cell types or even plants where they normally do not express.
Multiple enzymatic steps can transform the co-transported fumarate into phosphoenolpyruvate, which then can be used for gluconeogenesis.
Glutamate can also be converted to glutathione-conjugated products through multiple enzymatic steps with the final step being mediated by glutathione S-transferase (GST).
Bile acids (BAs) are synthesized from cholesterol through multiple enzymatic steps [ 5].
Glycosylation of the PG occurs in the Golgi apparatus in multiple enzymatic steps.
The cholesterol biosynthetic pathway comprises multiple enzymatic steps leading to cholesterol biosynthesis and these genes are coordinately up-regulated by IGF-1 (Fig. 2 and Table 4).
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Since fatty acid biosynthesis plays a central role in energy metabolism of all living organisms and involves multiple interplaying enzymatic steps [ 11- 17], its regulation is highly complicated and hence difficult to be manipulated.
In this regard, using supplementation with vitamin D3 in an intervention study to induce a clinical outcome that is mediated by the interaction between 1,25(OH)2D and the VDR (such as RAS inhibition to influence renal-vascular function) is analogous to administering a pre- or pro-drug that is dependent on multiple subsequent enzymatic steps, each regulated and modified by genetics and environment.
Thus multiple enzymatic and experimental steps were conducted before sequencing, each accounting for potential loss of the spike.
Traditional standard BioBrick assembly as well as 3A BioBrick assembly involves multiple enzymatic reactions and purification steps.
However, the library preparation methods required to render immunoprecipitated DNA ready for HTS sequencing involve inefficient enzymatic steps and multiple purifications, each resulting in sample loss.
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multiple enzymatic turnovers
multiple enzymatic mechanisms
multiple enzymatic specificities
multiple enzymatic events
multiple enzymatic functions
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multiple enzymatic processes
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multiple enzymatic complexes
multiple enzymatic pathways
multiple synthetic steps
multiple enzymatic systems
multiple intricate steps
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