Sentence examples for multiple entry clones from inspiring English sources

In the conventional strategy for gateway cloning, the construction of two or more expression elements into tandem elements on a single plasmid requires the recombination of multiple entry clones with a destination vector in a single reaction mixture.

To cover a large test space of ABA sensory domains, we engineered sensor constructs using >50 sPAS and dPAS variants sandwiched between a fluorescent protein FRET pair (i.e., multiple PAS Entry clones were recombined with a single destination vector. > -wrap-foot> 10.7554/eLiFigure41.004 Figure 1.

Use of increasing numbers of entry clones in a single reaction is inefficient due to the difficulty in successfully recognizing multiple pairs of matched att signals simultaneously.

The entry clones allow for a broad range of subsequent applications to functionally characterize the ORF encoded proteins in multiple expression systems in parallel [ 1, 13, 14, 23, 24].

Multiple entry by SMS is permitted, only one online entry per person.

Most career paths have multiple entry points.

All Entry clones were verified by restriction analysis and sequencing.

ORFs entry clones were amplified (all primers described in Table S3) from clones previously described.

These two entry clones were assembled with a 3' entry clone containing egfp and a destination vector containing the sC marker [5] through a recombination reaction.

The PCR products were cloned by recombination into pDONR221 (Invitrogen) using BP Clonase™ (Invitrogen), to generate the entry clones.

All the reactions were performed following the product protocol, and all the Entry clones and Expression clones have been sequenced.