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As the rates of isolation of strains differ in different media versions, multiple enrichment protocols were suggested to ensure the isolation of different strains [ 52].
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When microsatellites are isolated using standard SSR enrichment protocols and primers designed at complementary repeated regions, amplification from multiple genomic sites can cause scoring difficulties that compromise their utility as markers.
Herein, we present a time-efficient and cost-effective computational protocol that incorporates our novel program DROMPA (DRaw and Observe Multiple enrichment Profiles and Annotation) for peak calling and visualization.
Microsatellite isolation protocols have advanced technically, however, and current enrichment protocols make it difficult to compare with previous studies.
The two enrichment protocols are considered to be complementary [ 39– 41].
Not all enrichment protocols identified all the samples as positive.
Both enrichment protocols were performed following manufacturers' instructions.
This article offers an overview of currently used enrichment protocols.
Many enrichment protocols have been tested to increase target enrichment efficiency [ 88].
Multiple enrichment strategies are currently in use for target enrichment [ 1].
Nine different enrichment protocols were evaluated including six different broth mediums (CASO, ITC, PSB, PBS, PBSMSB and PBSSSB).
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