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This is the first study on layout design of multiple engineering features using level-set functions (LSFs) and Boolean operations.
First, multiple engineering features of arbitrary shape are considered as basic design primitives and topology variation is achieved via the layout and shape optimization of the features.
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Taking advantage of the multiplexable genome engineering feature of the CRISPR/Cas9 system, we sought to generate different kinds of immunodeficient mouse strains by embryo co-microinjection of Cas9 mRNA and multiple sgRNAs targeting mouse B2m, Il2rg, Prf1, Prkdc, and Rag1.
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