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DCLHb appears to work through multiple endogenous systems and mechanisms, each of which probably interact.
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Thus, the protective effects observed after METH preconditioning are not solely dependent on changes in one specific biochemical or molecular pathway but on multiple endogenous protective systems working in concert.
The need to inhibit Irga6 (and other GKS proteins) activation on multiple endogenous membrane systems may explain why all three GMS proteins are required for full control of Irga6 and Irgb6 activation.
Multiple endogenous growth factor systems function in a complex interaction to promote MN survival during development (Gould & Oppenheim, 2004).
To achieve this, we developed a novel experimental system in which multiple endogenous MEK and MKK pathways were inhibited by LF and MEK1 or MEK2 signaling was rescued by expressing a mutant MEK that was engineered to be resistant to LF-mediated proteolysis.
Open image in new window Figure 2 Simultaneously transcriptional activation of multiple endogenous genes using either dLbCpf1-p300core or dLbCpf1-SunTag system with a single gRNA for each gene.
Simultaneous activation of multiple endogenous genes with defined stoichiometry opens up novel opportunities for systems biology as it allows for the predictable manipulation of transcriptional networks.
In this study we report the generation of an RNA-programmable CRISPR-on system, which enables the simultaneous activation of multiple endogenous genes with a defined stoichiometry.
The C. elegans sensory system is exceptionally well suited for heterologous GPCR expression given that each neuron expresses multiple endogenous GPCRs [ 2].
Coupling of dCas9 to a transcriptional repressor domain can robustly silence expression of multiple endogenous genes.
Diurnal control is mediated by multiple endogenous and exogenous mechanisms, even at the level of individual genes.
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