However, the heterogeneous nature of the swollen vesicles indicates that they are probably derived from multiple compartments, and electron microscopic analysis supported this (supplementary Fig S4 online).
Electron microscopic studies demonstrated characteristic multiple membrane-bound inclusions within the eccrine epithelial cells, containing curvilinear inclusion material characteristic of neuronal ceroid lipofuscinosis.
For electron microscopic analysis, a Hitachi H-7000 electron microscope (Hitachi, Japan) was used.
Moreover, we showed the beneficial use of QDs for pre-embedding electron microscopic labeling, a technique now allowing labeling of multiple proteins and detection in EM-tomograms (manuscript submitted).
Scanning electron microscopic (SEM) measurements were performed on a Philips XL30 electron microscope (Philips, Amsterdam, The Netherlands).
Electron microscopic examination was conducted by using a Tecnai 10 electron microscope (Philips, Amsterdam, the Netherlands).
Scanning electron microscopic pictures were taken with a Zeiss SEM/FIB scanning electron microscope.
Electron microscopic data were then acquired using a Hitachi H-7650 transmission electron microscope.
Microscope settings are listed in Supplementary Table 4. Electron microscopic analysis was done as previously described17.
Scanning electron microscopic (EM) analysis of cells treated with compound 1 showed that the aberrant cells grew multiple new flagella, accompanied by the ingression of various cleavage furrows (Fig. 3D).
