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Recent studies have revealed that Ft's effects on distinct pathways may be genetically separated, and that multiple effector domains can contribute to the same function.
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Indeed, mutations of chromatin effector domains found within several proteins are associated with multiple human pathologies, including cancer and immunodeficiency syndromes.
Their modular structure enables both the sequential assembling of multiple ZFs to generate DBDs with different target specificities and the use of various effector domains to engineer ATFs or nucleases.
The effector domains of human NLRs are structurally variable, which result in the activation of multiple signaling pathways and biological functions [ 64].
AVEC consist of the effector domains: HBsAg, FcR-BD, C1q-BD and the targeting domains: anti-HER-2 CDRs.
The effector domains constitute: hepatitis B surface antigen (HBsAg); constant fragment receptor binding domain (FcR-BD); complement system binding domain (CS-BD).
PAS sensors detect chemical and physical stimuli and regulate the activity of functionally diverse effector domains.
Similarly, synthetic TFs can be constructed using a variety of DNA binding domains (DBDs) and effector domains.
Antibody x vaccine engineered construct (AVEC): anti-HER-2 x HBsAg consists of the targeting and effector domains.
Variations thereof lie mainly within the effector domains, additional domains and within the length of the linker from the effector domain to the NACHT domain.
In particular effector domains are subject to domain accretion, and/or domain shuffling or duplication for the acquisition of new domain architecture.
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