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Multiple drug effect analysis was performed using computer software as previously described [ 33].
Multiple drug effect analysis was carried out as previously described (Pegram et al, 2004).
Drug combinations were evaluated using CalcuSyn (BIOSOFT, Ferguson, MO) software based on the multiple drug effect equation of Chou-Talalay.
Multiple drug effect analysis was performed using computer software (Biosoft) as described in detail (Pegram et al, 2004).
Determination of synergy or antagonism was based on the multiple drug effect equation of Chou and Talalay (1977, 1983) and was quantified by the combination index (CI).
The compound interactions were calculated by multiple drug effect analysis and were performed by the median equation principle according to the methodology described by Chou and Talalayb (1984).
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The interaction between IGF-1Rki and PARP inhibitor was assessed using the multiple drug effects analysis method of Chou and Talalay [ 37].
Further, to determine the nature of the interaction between IGF-1Rki and PARP inhibitor we used the multiple drug effects analysis method of Chou and Talalay (see Materials and Methods) [ 37].
Multiple drug-effect analysis revealed strong synergistic effects in all the treatments.
Multiple drug-effect analysis was performed for combinations of Nutlin-3a with Doxorubicin, Cisplatin or Methotrexate using the CalcuSyn software.
This uses the multiple drug-effect equation of Chou and Talalay (1977) derived from enzyme kinetic models.
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