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In order to improve vaccine efficacy and safety of the ΔsecA2 mutant, we generated multiple double and triple mutants of the ΔsecA2 mutant strain via incorporation of other attenuating mutations of M. tuberculosis.
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Importantly, by multiple double- and triple immunogold labellings, it was shown that a particular early endosome-associated tubule could contain the recycling proteins Tf and ASGPR, the CD and CI-MPR, and LAMP-1 and LAMP-2.
The results of investigations using a fluoride-rich pyrazole, fluoro-ethylene carbonate, trially cyanurate with multiple double bonds, and LiF explain the combined action of the protective layer and fluoride in promoting longer life cycles.
The overreaction results in DMDS reacting with multiple double bonds, and the possible formation of intermolecular linkages, creating non-volatile products.
Febendazole and Riboflavin showed a low binding affinity to 482G, mainly due to the presence of multiple double bonds and aromatic rings.
At physiological temperatures, highly hydrophobic and non-polar cholesterol and lipids with high Tm, usually with fully saturated acyl chains, pack unfavorably with lipids with low Tm because, under physiological conditions, lipids with low Tm usually contain multiple double bonds and are more polar.
Indeed, electron microscopy confirms that tumor cells treated with PET-16, like PES, contain multiple double-membrane and single-membrane structures and multiple vacuoles, consistent with impaired autophagy.
Our investigation started with a Multiple Double Couples (MDC) analysis (Li and Ji, 2010).
Multiple double bond configurations are separated by semicolons and are listed with increasing values of x 1.
Intrigued by these results, we examined the chemoselectivity and regioselectivity with substrates bearing multiple double bonds.
The only other multiple double gold medalists were Eugenio Monti of Italy (1960 , 1961 1968) and Anderl Ostler of Germany (1951, 1952).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com